The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis .
Taotao YueJinpeng WangFang LiuPengtao GongJianhua LiXichen ZhangNan ZhangPublished in: Artificial cells, nanomedicine, and biotechnology (2024)
Lung cancer is a dangerous disease that is lacking in an ideal therapy. Here, we evaluated the anti-lung cancer effect in nude mice of a fully human single-chain antibody (scFv) against the associated antigen 7 transmembrane receptor (Ts7TMR), which is also called G protein-coupled receptor, between A549 cells and Trichinella spiralis ( T. spiralis ). Our data showed that anti-Ts7TMR scFv could inhibit lung cancer growth in a dose-dependent manner, with a tumour inhibition rate of 59.1%. HE staining did not reveal any obvious tissue damage. Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer.
Keyphrases
- endothelial cells
- induced apoptosis
- cell proliferation
- vascular endothelial growth factor
- type diabetes
- oxidative stress
- transcription factor
- stem cells
- metabolic syndrome
- dna methylation
- genome wide
- high fat diet induced
- cell death
- mesenchymal stem cells
- bone marrow
- endoplasmic reticulum stress
- insulin resistance
- adipose tissue
- deep learning
- flow cytometry
- pi k akt