Finite-difference time-domain to screen Au NPs as SERS active substrate for the sensitive determination of prohibited drugs in fish via solvent cleaning.
Shenxuan DuBorong YuFangwei HanYue MaoHuijiao ZhangJiangli LiPublished in: Analytical methods : advancing methods and applications (2022)
Surface enhanced Raman spectroscopy (SERS), as a molecule-specific method using plasmonic nanostructures to significantly enhance signal intensity, has been employed in various fields. In our study, we investigated the size effect of gold nanoparticles (Au NPs) on surface plasmon response by finite-difference time-domain (FDTD) simulation. In addition, SERS experiments, using the same concentrations of crystal violet (CV), were also carried out to confirm the simulation results. On this basis, the size of citrate-stabilized Au NPs (∼100 nm) was controlled by a seed-mediated growth, thus providing great electromagnetic field enhancement for SERS detection of CV in fish. Methanol solvent cleaning along with high speed centrifugal separation was developed, which could not only remove lipids in fish, but also produce hot spots via induced aggregation of Au NPs. The SERS detection limit of CV in fish could be less than 1 ppb. Such cost-effective and facile routes will be attractive for the trace detection of various analytes in complex matrices.
Keyphrases
- sensitive detection
- gold nanoparticles
- loop mediated isothermal amplification
- raman spectroscopy
- reduced graphene oxide
- label free
- quantum dots
- high speed
- atomic force microscopy
- real time pcr
- oxide nanoparticles
- high throughput
- visible light
- high frequency
- high resolution
- heavy metals
- drug induced
- high glucose
- risk assessment
- solid phase extraction