Daratumumab interference in flow cytometric anti-granulocyte antibody testing can be overcome using non-human blocking antibodies.
Nisar A BaigBrian A DukekDeborah K FalboLaurie L WakefieldMargaret A DiGuardoAleh BobrJustin D KreuterManish J GandhiPublished in: Vox sanguinis (2020)
We established that DARA can interfere with AGA testing, leading to false positive Flow-GIFT results without causing GAT agglutination. DTT treatment increased background binding of secondary antibodies causing a decrease in Flow-GIFT sensitivity. In comparison, blockage of the DARA binding epitope using mouse anti-CD38 antibody was effective in neutralizing DARA interference while maintaining Flow-GIFT sensitivity.