Unusual carriage of virulence genes sasX/sesI/shsA by nosocomial Staphylococcus haemolyticus from Brazil.
Ana C Silva-de-JesusCiro César RossiPaula Ma Pereira-RibeiroAna Luíza Mattos-GuaraldiMarcia Giambiagi-deMarvalPublished in: Future microbiology (2023)
Background: Staphylococcus haemolyticus is an emerging threat in the nosocomial environment but only some virulence factors are known. Materials & methods: The frequency of the sasX gene (or orthologues sesI/shsA ), encoding an invasiveness-related surface-associated protein, in S. haemolyticus was detected in different hospitals in Rio de Janeiro. Results: 9.4% of strains were sasX/sesI/shsA -positive, some were in the context of the ΦSPβ-like prophage and devoid of CRISPR systems, indicating potential transferability of their virulence genes. Gene sequencing evidenced that Brazilian S. haemolyticus harbored sesI , instead of the usual sasX , while S. epidermidis had sasX instead of sesI , suggesting horizontal acquisition. Conclusion: The contexts of Brazilian sasX/sesI/shsA favor transfer, which is alarming given the difficulty in treating infections caused by S. haemolyticus .
Keyphrases
- biofilm formation
- staphylococcus aureus
- genome wide
- escherichia coli
- pseudomonas aeruginosa
- genome wide identification
- candida albicans
- antimicrobial resistance
- methicillin resistant staphylococcus aureus
- dna methylation
- copy number
- acinetobacter baumannii
- klebsiella pneumoniae
- bioinformatics analysis
- genome wide analysis
- cystic fibrosis
- single cell
- transcription factor
- gene expression
- crispr cas
- climate change
- healthcare
- risk assessment