Dactylides A-C, three new bioactive 22-membered macrolides produced by Dactylosporangium aurantiacum.
Pankaj KumarYedukondalu NalliSanju SinghPadmaja D WakchaureRavi GorVishal A GhadgeEunji KimSatish RamalingamV N Azger DusthackeerYeo Joon YoonBishwajit GangulyPramod B ShindePublished in: The Journal of antibiotics (2023)
Three new 22-membered polyol macrolides, dactylides A-C (1-3), were isolated from Dactylosporangium aurantiacum ATCC 23491 employing repeated chromatographic separations, and their structures were established based on detailed analysis of NMR and MS data. The relative configurations at the stereocenters were established via vicinal 1 H- 1 H coupling constants, NOE correlations, and by application of Kishi's universal NMR database. In order to get insights into the biosynthetic pathway of 1-3, the genome sequence of the producer strain D. aurantiacum was obtained and the putative biosynthetic gene cluster encoding their biosynthesis was identified through bioinformatic analysis using antiSMASH. Compounds 1-3 showed significant in-vitro antimycobacterial and cytotoxic activity.
Keyphrases
- high resolution
- magnetic resonance
- solid state
- mass spectrometry
- multiple sclerosis
- electronic health record
- genome wide
- ms ms
- simultaneous determination
- adverse drug
- genome wide identification
- cell wall
- emergency department
- gene expression
- dna methylation
- machine learning
- liquid chromatography
- transcription factor
- tandem mass spectrometry