Enhancing the Quality of Antibodies to HIV-1 Envelope by GagPol-Specific Th Cells.
Michael Storcksdieck genannt BonsmannThomas NiezoldVladimir TemchuraFranco PissaniKatrin EhrhardtEric P BrownNana Yaw Osei-OwusuDrew HannamanHartmut HengelMargaret E AckermanHendrik StreeckGhulam NabiMatthias TenbuschKlaus ÜberlaPublished in: Journal of immunology (Baltimore, Md. : 1950) (2015)
The importance of Fc-dependent effector functions of Abs induced by vaccination is increasingly recognized. However, vaccination of mice against HIV envelope (Env) induced a skewed Th cell response leading to Env-specific Abs with reduced effector function. To overcome this bias, GagPol-specific Th cells were harnessed to provide intrastructural help for Env-specific B cells after immunization with virus-like particles containing GagPol and Env. This led to a balanced Env-specific humoral immune response with a more inflammatory Fc glycan profile. The increased quality in the Ab response against Env was confirmed by FcγR activation assays. Because the Env-specific Th cell response was also biased in human vaccinees, intrastructural help is an attractive novel approach to increase the efficacy of prophylactic HIV Env-based vaccines and may also be applicable to other particulate vaccines.
Keyphrases
- immune response
- antiretroviral therapy
- hiv positive
- hiv infected
- human immunodeficiency virus
- induced apoptosis
- dendritic cells
- hiv testing
- hiv aids
- endothelial cells
- single cell
- stem cells
- adipose tissue
- regulatory t cells
- skeletal muscle
- men who have sex with men
- high throughput
- metabolic syndrome
- cell proliferation
- endoplasmic reticulum stress
- type iii
- high fat diet induced