Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa.
Kun JiangXiaoyue HouLu HanTongtong TanZhanglei CaoJun CaiPublished in: Toxins (2018)
Vegetative insecticidal proteins (Vips), which are secreted by some Bacillus thuringiensis strains during vegetative growth, exhibit high virulence to many pests. Vip3A proteins have been used commercially both in some bio-insecticides and in transgenic crops; however, compared with insecticidal crystal proteins, the mechanism of action of Vip3A is still unclear. In this work, we indicated that the fibroblast growth factor receptor-like protein (Sf-FGFR) from the membrane of Sf9 cells could bind to Vip3Aa. The interaction between Vip3Aa and Sf-FGFR was confirmed by pull-down assays and dot blotting experiment in vitro. The binding affinity between Vip3Aa and extracellular regions of Sf-FGFR (GST-FGFR-N) was determined by microscale thermophoresis assay (MST). Moreover, Vip3Aa-Flag could be co-immunoprecipitated with Sf-FGFR-V5 ex vivo. Furthermore, knockdown of Sf-FGFR gene in Sf9 cells resulted in reducing the mortality of those cells to Vip3Aa. In summary, our data indicated that Sf-FGFR is a novel receptor for Vip3Aa.
Keyphrases
- induced apoptosis
- cell cycle arrest
- escherichia coli
- high throughput
- cell death
- endoplasmic reticulum stress
- gene expression
- staphylococcus aureus
- cardiovascular events
- machine learning
- pseudomonas aeruginosa
- signaling pathway
- big data
- type diabetes
- genome wide
- cardiovascular disease
- oxidative stress
- transcription factor
- copy number
- risk factors
- artificial intelligence
- electronic health record
- capillary electrophoresis
- aedes aegypti