A connectome and analysis of the adult Drosophila central brain.
Louis K SchefferC Shan XuMichał JanuszewskiZhiyuan LuShin-Ya TakemuraKenneth J HayworthGary B HuangKazunori ShinomiyaJeremy Maitlin-ShepardStuart BergJody ClementsPhilip M HubbardWilliam T KatzLowell UmayamTing ZhaoDavid AckermanTim BlakelyJohn A BogovicTom DolafiDagmar KainmuellerTakashi KawaseKhaled A KhairyLaramie LeavittPeter H LiLarry LindseyNicole NeubarthDonald J OlbrisHideo OtsunaEric T TrautmanMasayoshi ItoAlexander Shakeel BatesJens GoldammerTanya WolffRobert R SvirskasPhilipp SchlegelErika NeaceChristopher J KnechtChelsea X AlvaradoDennis A BaileySamantha BallingerJolanta A BoryczBrandon S CaninoNatasha CheathamMichael CookMarisa DreherOctave DuclosBryon EubanksKelli FairbanksSamantha FinleyNora ForknallAudrey FrancisGary Patrick HopkinsEmily M JoyceSungJin KimNicole A KirkJulie KovalyakShirley A LauchieAlanna LohffCharli MaldonadoEmily A ManleySari McLinCaroline MooneyMiatta NdamaOmotara OgundeyiNneoma OkeomaChristopher OrdishNicholas PadillaChristopher M PatrickTyler PatersonElliott E PhillipsEmily M PhillipsNeha RampallyCaitlin RibeiroMadelaine K RobertsonJon Thomson RymerSean M RyanMegan SammonsAnne K ScottAshley L ScottAya ShinomiyaClaire SmithKelsey SmithNatalie L SmithMargaret A SobeskiAlia SuleimanJackie SwiftSatoko TakemuraIris TalebiDorota TarnogorskaEmily TenshawTemour TokhiJohn J WalshTansy YangJane Anne HorneFeng LiRuchi ParekhPatricia K RivlinVivek JayaramanMarta CostaGregory S X E JefferisKei ItoStephan SaalfeldReed GeorgeIan A MeinertzhagenGerald M RubinHarald F HessViren JainStephen M PlazaPublished in: eLife (2020)
The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction of the brain of the fruit fly Drosophila melanogaster. Improved methods include new procedures to prepare, image, align, segment, find synapses in, and proofread such large data sets. We define cell types, refine computational compartments, and provide an exhaustive atlas of cell examples and types, many of them novel. We provide detailed circuits consisting of neurons and their chemical synapses for most of the central brain. We make the data public and simplify access, reducing the effort needed to answer circuit questions, and provide procedures linking the neurons defined by our analysis with genetic reagents. Biologically, we examine distributions of connection strengths, neural motifs on different scales, electrical consequences of compartmentalization, and evidence that maximizing packing density is an important criterion in the evolution of the fly's brain.