Prediction the Molecular Mechanism of Shengmai Injection in Acute Treatment of COVID-19 Based on Network Pharmacology.
Chen WangAo-Lei LiuHe-Zhen WuYan-Fang YangPublished in: Natural product communications (2022)
Objective: To predict the mechanism of Shengmai Injection (SMI) in the acute treatment of COVID-19 by network pharmacology and molecular docking. Methods: Search the compounds in the Traditional Chinese Medicine Systems Pharmacology (TCMSP), and screen them by Drug-like properties (DL) and Oral bioavailability (OB); Using PharmMapper database and GeneCards database to collect compounds targets and COVID-19 targets, and using UniProt database to standardize the names of target genes; Using DAVID database for KEGG pathway annotation and GO bioinformatics analysis; Using Cytoscape 3.8.2 software and STRING 10.5 database to construct "Component-Target-Pathway" network and Protein-Protein Interaction network (PPI); Using molecular docking to predict the binding ability of key compounds and key proteins. Results: A total of 34 active components, 38 core targets and 180 signaling pathways were screened out. The results of molecular docking showed that Schisantherin A and Moupinamide have strong binding with EGFR and MAPK1. Conclusion: The key active compounds of SMI in the treatment of COVID-19 may be Schisantherin A and Moupinamide, and the molecular mechanism may be related to key targets such as EGFR and MAPK1, and may be involved in the PI3K-Akt signaling pathway and MAPK signaling pathway.
Keyphrases
- molecular docking
- signaling pathway
- coronavirus disease
- sars cov
- pi k akt
- molecular dynamics simulations
- small cell lung cancer
- adverse drug
- protein protein
- liver failure
- epithelial mesenchymal transition
- oxidative stress
- induced apoptosis
- emergency department
- small molecule
- tyrosine kinase
- epidermal growth factor receptor
- drug induced
- respiratory syndrome coronavirus
- genome wide
- dna methylation
- combination therapy
- ultrasound guided
- gene expression
- acute respiratory distress syndrome