A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO- near NMDA Receptors in Neuronal Cells and Hippocampal Tissues.
Dayoung LeeChang Su LimGyeongju KoDayoung KimMyoung Ki ChoSang-Jip NamHwan Myung KimJuyoung YoonPublished in: Analytical chemistry (2018)
In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO-) near the N-methyl-d-aspartate (NMDA) receptor. This naphthalimide-based probe contains a boronic acid reactive group and an ifenprodil-like tail, which serves as an NMDA receptor targeting unit. The probe displays high sensitivity and selectivity, along with a fast response time in aqueous solution. More importantly, the probe can be employed along with two-photon fluorescence microscopy to detect endogenous ONOO- near NMDA receptors in neuronal cells as well as in hippocampal tissues. The results suggest that the probe has the potential of serving as a useful imaging tool for studying ONOO- related diseases in the nervous system.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- induced apoptosis
- high resolution
- cerebral ischemia
- aqueous solution
- cell cycle arrest
- gene expression
- endoplasmic reticulum stress
- signaling pathway
- oxidative stress
- high throughput
- cancer therapy
- cell death
- label free
- subarachnoid hemorrhage
- quantum dots
- climate change
- single cell