Microsatellite Instability Detection in Cancer: A Multiplex qPCR Approach that Obviates the Need for Matching Normal Samples.

Wei ChenYan Helen YanBlake YoungAlessandro PintoQi JiangNanjia SongAdam YaseenWeijie YaoDavid Yu ZhangJinny Xuemeng Zhang

Published in: Clinical chemistry (2024)

This study validates VarTrace as a swift, accurate, and economical assay for MSI detection in samples with low tumor percentages without the need for matching normal DNA. VarTrace's capacity for highly sensitive MSI analysis holds potential for enhancing the efficiency of clinical work flows and broadening the availability of this test.

Keyphrases

real time pcr
label free
high throughput
loop mediated isothermal amplification
papillary thyroid
circulating tumor
quantum dots
living cells