Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification.
Yanchen ChiMeng ShiYanfang WuYunping WuYangyang ChangMeng LiuPublished in: Analytical methods : advancing methods and applications (2022)
We described a new system termed droplet DNAzyme-coupled rolling circle amplification (dDRCA) that can selectively detect bacteria from clinical urine samples with single-cell sensitivity within 1.5 h compared with the several hours needed for traditionally used culture-based methods.