Effects of lyoprotectants on long-term stability and transfection efficacy of lyophilized poly(lactide-co-glycolide)-graft-polyethylenimine/plasmid DNA polyplexes.
Joshua WooJeoung Soo LeePublished in: Nanomedicine (London, England) (2021)
Aim: We investigated the effect of lyoprotectants on the long-term stability and transfection efficiency of lyophilized (Lyo.) polyplexes prepared from poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) and plasmid DNA encoding green fluorescent protein (pGFP). Materials & methods: Lyo. PgP/pGFP polyplexes prepared with/without lyoprotectants were stored at -20°C over 6 months. Polyplex stability was analyzed by gel electrophoresis and heparin competition assay. Transfection efficiency and cytotoxicity were evaluated in rat glioma (C6) cells in medium containing 10% serum. Results: Lyo. PgP/pGFP polyplexes prepared with 5% sucrose as a lyoprotectant remained stable up to 6 months and retained transfection efficiency up to 4 months. Conclusion: Lyo. PgP-based polyplexes retain bioactivity during extended storage, potentially enabling transport to remote regions and less stable settings, increasing access to life-changing gene therapy.
Keyphrases
- gene therapy
- escherichia coli
- circulating tumor
- induced apoptosis
- single molecule
- crispr cas
- cell free
- venous thromboembolism
- oxidative stress
- cell cycle arrest
- high throughput
- living cells
- room temperature
- growth factor
- endoplasmic reticulum stress
- cell death
- binding protein
- high resolution
- circulating tumor cells
- single cell
- ionic liquid
- protein protein