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Versatile Near-Infrared Super-Resolution Imaging of Amyloid Fibrils with the Fluorogenic Probe CRANAD-2.

Joaquim TorraFelipe VielaDiego MegíasBegoña SotCristina Flors
Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
CRANAD-2 is a fluorogenic curcumin derivative used for near-infrared detection and imaging in vivo of amyloid aggregates, which are involved in neurodegenerative diseases. We explore the performance of CRANAD-2 in two super-resolution imaging techniques, namely stimulated emission depletion (STED) and single-molecule localization microscopy (SMLM), with markedly different fluorophore requirements. By conveniently adapting the concentration of CRANAD-2, which transiently binds to amyloid fibrils, we show that it performs well in both techniques, achieving a resolution in the range of 45-55 nm. Correlation of SMLM with atomic force microscopy (AFM) validates the resolution of fine features in the reconstructed super-resolved image. The good performance and versatility of CRANAD-2 provides a powerful tool for near-infrared nanoscopic imaging of amyloids in vitro and in vivo.
Keyphrases
  • single molecule
  • atomic force microscopy
  • high resolution
  • high speed
  • living cells
  • photodynamic therapy
  • high throughput
  • quantum dots
  • single cell
  • fluorescent probe