In vitro inhibitory effect of zingerone on TNFα-stimulated fibroblast-like synoviocytes.
Yuhang MaoChangze LiuDan LiuXianhua WeiXin TanJunnan ZhouXiaolu YuMei LiuPublished in: In vitro cellular & developmental biology. Animal (2023)
Targeting Fibroblast-like synoviocytes (FLSs) is an attractive complementary approach for RA therapy. This study aimed to investigate the inhibitory effects of zingerone on TNFα-induced arthritic FLSs. MTS, EdU, wound healing, DHE staining and real-time PCR were used to determine the effects of zingerone on the destructive behaviors of arthritic FLSs induced by TNFα. Western blot analysis was used to analyze cell signaling pathways. Zingerone treatment significantly inhibited TNFα-induced proliferation, migration, ROS formation and pro-inflammatory cytokines expression of FLSs. Molecular mechanism studies revealed that zingerone could suppress TNFα-induced activations of MAPKs (ERK, JNK and p38) in arthritic FLSs. Zingerone attenuated pathological features of FLSs via MAPKs pathways, indicating its potential as a complementary or alternative drug for RA therapy.
Keyphrases
- rheumatoid arthritis
- signaling pathway
- high glucose
- diabetic rats
- wound healing
- drug induced
- disease activity
- single cell
- real time pcr
- cell proliferation
- cell death
- stem cells
- endothelial cells
- emergency department
- oxidative stress
- pi k akt
- south africa
- bone marrow
- cancer therapy
- interstitial lung disease
- cell therapy
- systemic sclerosis
- idiopathic pulmonary fibrosis
- reactive oxygen species
- combination therapy
- mesenchymal stem cells
- case control
- binding protein
- flow cytometry