Rapid MALDI mass spectrometry imaging for surgical pathology.
Sankha S BasuMichael S ReganElizabeth C RandallWalid M AbdelmoulaAmanda R ClarkBegoña Gimenez-Cassina LopezDale S CornettAndreas HaaseSandro SantagataNathalie Y R AgarPublished in: NPJ precision oncology (2019)
Matrix assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) is an emerging analytical technique, which generates spatially resolved proteomic and metabolomic images from tissue specimens. Conventional MALDI MSI processing and data acquisition can take over 30 min, limiting its clinical utility for intraoperative diagnostics. We present a rapid MALDI MSI method, completed under 5 min, including sample preparation and analysis, providing a workflow compatible with the clinical frozen section procedure.
Keyphrases
- mass spectrometry
- high resolution
- liquid chromatography
- gas chromatography
- high performance liquid chromatography
- capillary electrophoresis
- electronic health record
- tandem mass spectrometry
- deep learning
- simultaneous determination
- loop mediated isothermal amplification
- convolutional neural network
- optical coherence tomography
- fluorescence imaging
- label free