Iridoids from the Roots of Patrinia scabra and Their Inhibitory Potential on LPS-Induced Nitric Oxide Production.
Da Hye LeeJi-Sun ShinShin-Young KangSeung-Bin LeeJin Su LeeSeung Mok RyuKyung Tae LeeDongho LeeDae-Sik JangPublished in: Journal of natural products (2018)
An activity-guided fractionation procedure of the 70% aqueous EtOH extract from the roots of Patrinia scabra led to the isolation and characterization of five new iridoids, patriscabrins A-E (1-5), along with 13 known compounds. The structures of 1-5 were determined by interpretation of spectroscopic data, particularly by 1D and 2D NMR, ECD, and VCD studies. Thereafter, isolates were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells. Of these, the new iridoids 2 and 5 and the known lignan patrineolignan B (6) exhibited IC50 values of 14.7 to 17.8 μM.
Keyphrases
- lipopolysaccharide induced
- inflammatory response
- nitric oxide
- lps induced
- induced apoptosis
- high resolution
- nitric oxide synthase
- hydrogen peroxide
- cell cycle arrest
- oxidative stress
- molecular docking
- electronic health record
- minimally invasive
- big data
- ionic liquid
- anti inflammatory
- cell proliferation
- case control
- signaling pathway
- human health
- climate change
- genetic diversity