Phytochemistry and Antihyperglycemic Potential of Cistus salviifolius L., Cistaceae.
Maja HitlKatarina BijelićNebojša P StilinovićBiljana BožinBranislava Srđenović ČonićLjilja TorovićNebojša KladarPublished in: Molecules (Basel, Switzerland) (2022)
Cistus salviifolius has been previously reported as a traditional remedy for hyperglycemia. However, the plant has been scarcely investigated from scientific point of view. Thus, the aim was to examine the chemical composition and to evaluate its antioxidant and antihyperglycemic potential in vitro. Aqueous and ethanolic extracts were evaluated for total phenolic, tannin, and flavonoid content using spectrophotometric methods. Detailed chemical characterization was performed by high-performance liquid chromatography (HPLC-DAD). The volatile organic compounds (VOCs) profile was assessed by gas chromatography technique. The potential in diabetes treatment was evaluated through tests of free radicals neutralization, inhibition of lipid peroxidation process, and test of ferric ion reduction; activity in tests of inhibition of α-amylase, α-glucosidase and dipeptidyl peptidase-4 was also evaluated. High content of phenolics (majority being tannins) was detected; detailed HPLC analysis revealed high content of gallic acid, followed by rutin, chlorogenic and caffeic acids. The VOCs analysis determined sesquiterpene hydrocarbons and oxygenated sesquiterpenes as the main groups of compounds. The assays classified extracts as potent neutralizers of 2,2-diphenyl-1-picrylhydrazil and nitroso radicals formation and potent inhibitors of α-amylase and α-glucosidase. In conclusion, Cistus salviifolius represents a rich source of phenolics and essential oil with sesquiterpenes. The established results suggested its promising antioxidant and antihyperglycemic activities.
Keyphrases
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- gas chromatography
- mass spectrometry
- ms ms
- liquid chromatography
- oxidative stress
- essential oil
- anti inflammatory
- cardiovascular disease
- type diabetes
- high resolution mass spectrometry
- human health
- adipose tissue
- gas chromatography mass spectrometry
- single cell
- climate change
- ionic liquid
- combination therapy
- skeletal muscle
- metabolic syndrome
- atomic force microscopy
- single molecule
- replacement therapy
- data analysis