Development of a Fully Automated Desktop Analyzer and Ultrahigh Sensitivity Digital Immunoassay for SARS-CoV-2 Nucleocapsid Antigen Detection.

Ryotaro ChibaKei MiyakawaKotaro AokiTakamitsu J MorikawaYoshiki MoriizumiTakuma DegawaYoshiyuki AraiOsamu SegawaKengo TanakaHideji TajimaSusumu AraiHisatoshi YoshinagaRyohei TsukadaAkira TaniHaruhito FujiAkinobu SatoYoshikazu IshiiKazuhiro TatedaAkihide RyoToru Yoshimura

Published in: Biomedicines (2022)

Our d-IA system, which includes a novel fully automated desktop analyzer, enabled detection of the SARS-CoV-2 N-protein with a comparable sensitivity to RT-PCR within 30 min. Thus, d-IA shows potential for SARS-CoV-2 detection across multiple diagnostic centers including small clinics, hospitals, airport quarantines, and clinical laboratories.

Keyphrases

sars cov
respiratory syndrome coronavirus
real time pcr
label free
loop mediated isothermal amplification
deep learning
healthcare
risk assessment
coronavirus disease
small molecule
protein protein