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Development of a Fully Automated Desktop Analyzer and Ultrahigh Sensitivity Digital Immunoassay for SARS-CoV-2 Nucleocapsid Antigen Detection.

Ryotaro ChibaKei MiyakawaKotaro AokiTakamitsu J MorikawaYoshiki MoriizumiTakuma DegawaYoshiyuki AraiOsamu SegawaKengo TanakaHideji TajimaSusumu AraiHisatoshi YoshinagaRyohei TsukadaAkira TaniHaruhito FujiAkinobu SatoYoshikazu IshiiKazuhiro TatedaAkihide RyoToru Yoshimura
Published in: Biomedicines (2022)
Our d-IA system, which includes a novel fully automated desktop analyzer, enabled detection of the SARS-CoV-2 N-protein with a comparable sensitivity to RT-PCR within 30 min. Thus, d-IA shows potential for SARS-CoV-2 detection across multiple diagnostic centers including small clinics, hospitals, airport quarantines, and clinical laboratories.
Keyphrases
  • sars cov
  • respiratory syndrome coronavirus
  • real time pcr
  • label free
  • loop mediated isothermal amplification
  • deep learning
  • healthcare
  • risk assessment
  • coronavirus disease
  • small molecule
  • protein protein