Glutathione S -transferase gene polymorphism and asthma: a case-control study in a pediatric population.
Ankit KumarTanzeel AhmedMohammad Khalid ParvezBasu Dev BanerjeeMazher MaqusoodRajesh M JagirdarPublished in: Pharmacogenomics (2022)
Aim: To carry out a case-control study of the association of GST gene polymorphisms in pediatric asthma-related oxidative stress. Materials & methods: Asthma patients (n = 250) and age-matched healthy subjects (n = 250) DNA were genotyped for GSTM1/GSTT1 (+/+, +/-, -/+ and -/-) frequencies using multiplex-PCR and plasma oxidative stress markers (examined spectrophotometrically). Results: Asthma patients had significantly more common null-genotype GSTM1 - /GSTT1 - (10.4%; p = 0.002) and elevated levels of malondialdehyde, protein carbonyl and 8-hydroxy-2-deoxyguanosine as compared with controls. In addition, the level of plasma glutathione, GST activity and ferric-reducing ability were significantly decreased as compared with controls. Conclusion: Our data revealed significant associations between GSTM1 - /GSTT1 - genotype and oxidative stress markers in asthmatic children, which may very likely contribute to increased incidence of bronchial asthma.
Keyphrases
- oxidative stress
- chronic obstructive pulmonary disease
- lung function
- end stage renal disease
- ejection fraction
- newly diagnosed
- chronic kidney disease
- allergic rhinitis
- dna damage
- prognostic factors
- young adults
- induced apoptosis
- high throughput
- risk factors
- patient reported outcomes
- air pollution
- circulating tumor
- patient reported
- cell free
- binding protein