Blood Plasma Stabilized Gold Nanoclusters for Personalized Tumor Theranostics.
Greta JarockyteVilius PoderysVirginijus BarzdaVitalijus KarabanovasRicardas RotomskisPublished in: Cancers (2022)
Personalized cancer theranostics has a potential to increase efficiency of early cancer diagnostics and treatment, and to reduce negative side-effects. Protein-stabilized gold nanoclusters may serve as theranostic agents. To make gold nanoclusters personalized and highly biocompatible, the clusters were stabilized with human plasma proteins. Optical properties of synthesized nanoclusters were investigated spectroscopically, and possible biomedical application was evaluated using standard cell biology methods. The spectroscopic investigations of human plasma proteins stabilized gold nanoclusters revealed that a wide photoluminescence band in the optical tissue window is suitable for cancer diagnostics. High-capacity generation of singlet oxygen and other reactive oxygen species was also observed. Furthermore, the cluster accumulation in cancer cells and the photodynamic effect were evaluated. The results demonstrate that plasma proteins stabilized gold nanoclusters that accumulate in breast cancer cells and are non-toxic in the dark, while appear phototoxic under irradiation with visible light. The results positively confirm the utility of plasma protein stabilized gold nanoclusters for the use in cancer diagnostics and treatment.
Keyphrases
- papillary thyroid
- sensitive detection
- energy transfer
- fluorescent probe
- squamous cell
- label free
- silver nanoparticles
- single cell
- breast cancer cells
- squamous cell carcinoma
- radiation therapy
- lymph node metastasis
- stem cells
- high resolution
- visible light
- drug delivery
- mass spectrometry
- combination therapy
- bone marrow
- climate change
- amino acid
- young adults
- replacement therapy
- ionic liquid