Association of bla VIM-2 , bla PDC-35 , bla OXA-10,   bla OXA-488 and bla VEB-9 β-Lactamase Genes with Resistance to Ceftazidime-Avibactam and Ceftolozane-Tazobactam in Multidrug-Resistant Pseudomonas aeruginosa .

Ceftazidime-avibactam and ceftolozane-tazobactam are approved for the treatment of complicated Gram-negative bacterial infections including multidrug-resistant (MDR) Pseudomonas aeruginosa . Resistance to both agents has been reported, but the underlying mechanisms have not been fully explored. This study aimed to correlate β-lactamases with phenotypic resistance to ceftazidime-avibactam and/or ceftolozane-tazobactam in MDR- P. aeruginosa from Qatar. A total of 525 MDR- P. aeruginosa isolates were collected from clinical specimens between 2014 and 2017. Identification and antimicrobial susceptibility were performed by the BD Phoenix TM system and gradient MIC test strips. Of the 75 sequenced MDR isolates, 35 (47%) were considered as having difficult-to-treat resistance, and 42 were resistant to ceftazidime-avibactam (37, 49.3%), and/or ceftolozane-tazobactam (40, 53.3%). They belonged to 12 sequence types, with ST235 being predominant (38%). Most isolates (97.6%) carried one or more β-lactamase genes, with bla OXA-488 (19%) and bla VEB-9 (45.2%) being predominant. A strong association was detected between class B β-lactamase genes and both ceftazidime-avibactam and ceftolozane-tazobactam resistance, while class A genes were associated with ceftolozane-tazobactam resistance. Co-resistance to ceftazidime-avibactam and ceftolozane-tazobactam correlated with the presence of bla VEB-9 , bla PDC-35 , bla VIM-2 , bla OXA-10 and bla OXA-488 . MDR- P. aeruginosa isolates resistant to both combination drugs were associated with class B β-lactamases ( bla VIM-2 ) and class D β-lactamases ( bla OXA-10 ), while ceftolozane-tazobactam resistance was associated with class A ( bla VEB-9 ), class C ( bla VPDC-35 ), and class D β-lactamases ( bla OXA-488 ).