A new anti-inflammatory flavonoid glycoside from tetraena aegyptia.
Ahmed A ZakiXiaoqian XuYuewen WangPei-Hsin ShieLongxin QiuPublished in: Natural product research (2019)
The chromatographic reinvestigation the methanol extract of Tetraena aegyptia led to the separation of a new flavonoid glycoside, isorhamnetin-3-O-[2```,3```-O-isopropylidene-α-L-rhamnopyranosyl]-(1```→6``)-O-β-D-glucopyranoside (1), together with two known flavonoids, isorhamnetin (2) and isorhamnetin-3-O-glucoside (3), isolated for the first time from the plant. The new compound was evaluated for the anti-inflammatory activity by using LPS-induce RAW 264.7 cells model. Compound 1 showed significant inhibitory effect on NO release. ELISA assay showed a pronounced effect of 1 on the secretion of cytokines IL-6 and TNF-α, in a dose-dependent manner. Consistent results were obtained by qRT-PCR which revealed that compound 1 markedly reduced the mRNA expression of IL-6 and TNF-α. Together these data, we demonstrated the anti-inflammatory activity of compound 1.
Keyphrases
- anti inflammatory
- rheumatoid arthritis
- induced apoptosis
- oxidative stress
- inflammatory response
- cell cycle arrest
- electronic health record
- single cell
- cell proliferation
- machine learning
- mass spectrometry
- simultaneous determination
- high resolution
- artificial intelligence
- pi k akt
- endoplasmic reticulum stress
- monoclonal antibody