Lectins as Biomarkers of IC/BPS Disease: A Comparative Study of Glycosylation Patterns in Human Pathologic Urothelium and IC/BPS Experimental Models.
Dominika PeskarTadeja KuretJera JerucAndreja ErmanPublished in: Diagnostics (Basel, Switzerland) (2022)
Pathophysiology of interstitial cystitis/bladder pain syndrome (IC/BPS) remains poorly understood, as well as its effective diagnosis and therapy. Studying changes in tissue glycosylation patterns under pathological conditions is a promising way of discovering novel biomarkers and therapeutic targets. The glycobiology of IC/BPS is largely understudied, therefore we compared glycosylation patterns of normal human urothelium with the urothelium of IC/BPS patients using a selection of 10 plant-based lectins with different monosaccharide preferences. We also compared lectin binding to human urothelium with the two most cited experimental models of IC/BPS, specifically, TNFα-treated human urothelial cell line RT4 and cyclophosphamide-induced chronic cystitis in C57BL6/J mice. Furthermore, binding of four of the selected lectins (ConA, DSL, Jacalin and WGA) was evaluated qualitatively by means of fluorescence microscopy, and quantitatively by fluorescence intensity (F.I.) measurements. Our results reveal a significant reduction in F.I. of Jacalin, as well as a prominent change in the WGA labeling pattern in the urothelium of IC/BPS patients, suggesting their potential use as promising additional biomarkers for histopathological diagnosis of IC/BPS. We have also shown that urothelial glycosylation patterns between selected experimental models and patients with IC/BPS are similar enough to offer an adequate platform for preclinical study of IC/BPS glycobiology.
Keyphrases
- endothelial cells
- newly diagnosed
- end stage renal disease
- ejection fraction
- single molecule
- high glucose
- rheumatoid arthritis
- prognostic factors
- stem cells
- type diabetes
- high resolution
- adipose tissue
- peritoneal dialysis
- dna methylation
- metabolic syndrome
- squamous cell carcinoma
- oxidative stress
- high dose
- high throughput
- radiation therapy
- patient reported outcomes
- skeletal muscle
- high speed
- genome wide
- high fat diet induced
- quantum dots
- single cell