Green highly sensitive spectrofluorimetric method for rapid determination of tafluprost in its pure form and ophthalmic formulation.

A green, simple and easy spectrofluorimetric method was studied for rapid estimation of tafluprost (TFL). The native fluorescence of TFL was measured at 292 nm after excitation at 220 nm. The results were linear in water over the concentration range 50-600 ng ml-1 with a correlation coefficient r = 0.9999 and intercept 1.1555. The limit of detection and limit of quantification were found to be 7.87 and 23.86 ng ml-1 , respectively. Neither different pH nor surfactants enhanced the fluorescence intensity. The high sensitivity of this spectrofluorimetric method makes it suitable for analysis of low concentrations of tafluprost in commercially available ophthalmic formulations. This procedure was validated according to International Council for Harmonisation Guidelines.