Advantages of nanofibrous membranes for culturing of primary RPE cells compared to commercial scaffolds.

Lucie TichotováHana Studenovska Goran PetrovskiŠtěpán PopelkaYaroslav NemeshMiroslava SedláčkováSaskia DrutovičSonali RohiwalDaniel HorákSlaven ErcegAnna BrymováAna Artero-CastroLyubomyr LytvynchukZbyněk StraňákZdeňka EllederováJan MotlíkTaras Ardan

Published in: Acta ophthalmologica (2021)

Retinal pigment epitheliums cultured on polylactide nanofibrous membranes improved the final quality of the cell product by having better maturation and long-term survival of the RPE monolayer compared to those cultured on commercial polyester scaffolds. PDLLA-cultured RPEs are a plausible source for the replacement of non-functioning RPEs during cell therapy.

Keyphrases

cell therapy
tissue engineering
endothelial cells
stem cells
mesenchymal stem cells
induced apoptosis
cell cycle arrest
single cell
quality improvement
bone marrow
cell death
cell proliferation