Whole-Genome Sequencing of ST2 A. baumannii Causing Bloodstream Infections in COVID-19 Patients.
Sabrina CherubiniMariagrazia PerilliBernardetta SegatorePaolo FaziiGiustino ParrutiAntonella FrattariGianfranco AmicosanteAlessandra PiccirilliPublished in: Antibiotics (Basel, Switzerland) (2022)
A total of 43 A. baumannii strains, isolated from 43 patients affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and by bacterial sepsis, were analyzed by antimicrobial susceptibility testing. All strains were resistant to almost three different classes of antibiotics, including carbapenems and colistin. The whole-genome sequencing (WGS) of eight selected A. baumannii isolates showed the presence of different insertion sequences (ISs), such as IS Aba13 , IS Aba26 , IS 26 , IS Vsa3 , IS Ec29 , IS 6100 and IS 17, giving to A. baumannii a high ability to capture and mobilize antibiotic resistance genes. Resistance to carbapenems is mainly mediated by the presence of OXA-23, OXA-66 and OXA-82 oxacillinases belonging to OXA-51-like enzymes. The presence of AmpC cephalosporinase, ADC-25, was identified in all A. baumannii . The pathogenicity of A. baumannii was exacerbated by the presence of several virulence factors. The multi-locus sequence typing (MLST) analysis showed that all strains belong to sequence type 2 (ST) international clone.
Keyphrases
- klebsiella pneumoniae
- sars cov
- escherichia coli
- acinetobacter baumannii
- respiratory syndrome coronavirus
- pseudomonas aeruginosa
- multidrug resistant
- antibiotic resistance genes
- drug resistant
- end stage renal disease
- biofilm formation
- transcription factor
- newly diagnosed
- chronic kidney disease
- microbial community
- coronavirus disease
- ejection fraction
- genetic diversity
- prognostic factors
- magnetic resonance imaging
- antimicrobial resistance
- arabidopsis thaliana
- patient reported outcomes
- amino acid
- magnetic resonance
- patient reported
- diffusion weighted
- contrast enhanced
- diffusion weighted imaging