Humoral immune response to authentic circulating severe acute respiratory syndrome coronavirus 2 variants elicited by booster vaccination with distinct receptor-binding domain subunits in mice.
Yushan JiangHuan ZhangJianhai YuDong HuangLinlin ZhaiMengjun LiYuelin WangZuning RenLirong ZouZhonghua ZhengHuanyu HuJunfang ZhangBao ZhangWei ZhaoXingfen YangBaisheng LiChenguang ShenPublished in: Journal of medical virology (2022)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants could induce immune escape by mutations of the spike protein which are threatening to weaken vaccine efficacy. A booster vaccination is expected to increase the humoral immune response against SARS-CoV-2 variants in the population. We showed that immunization with two doses of wild type receptor-binding domain (RBD) protein, and booster vaccination with wild type or variant RBD protein all significantly increased binding and neutralizing antibody titers against wild type SARS-CoV-2 and its variants in mice. Only the booster immunization by Omicron (BA.1)RBD induced a strong antibody titer against the omicron virus strain and comparable antibody titers against all the other virus strains. These findings might shed the light on coronavirus disease 2019 booster immunogens.
Keyphrases
- wild type
- respiratory syndrome coronavirus
- sars cov
- immune response
- coronavirus disease
- binding protein
- copy number
- protein protein
- toll like receptor
- dendritic cells
- escherichia coli
- amino acid
- dna binding
- type diabetes
- drug induced
- skeletal muscle
- metabolic syndrome
- gene expression
- zika virus
- insulin resistance
- transcription factor
- high glucose
- adipose tissue
- diabetic rats
- dna methylation
- oxidative stress
- endothelial cells