A detection system using sensing motif-tethered oligodeoxynucleotides for multiplex biomolecular analysis.

Tatsuya NishiharaYuto MotohashiReoto MioMasato SugawaraKazuhito Tanabe

Published in: Chemical communications (Cambridge, England) (2024)

We developed a system to detect multiple target biomolecules through sensing motif-tethered oligodeoxynucleotides. DNA-based molecular probes gave the primary amine motif upon reaction with the target biomolecules, glutathione (GSH) and H 2 O 2 . After labelling with biotin, the product DNAs were selectively collected to be quantified by qPCR.

Keyphrases

single molecule
real time pcr
high throughput
living cells
fluorescent probe
fluorescence imaging
photodynamic therapy
quantum dots
electron transfer
sensitive detection