Functional validation of A2'N mutation of the RDL GABA receptor against fipronil via molecular modeling and genome engineering in Drosophila.

This study confirmed that A2'N mutation of the RDL GABA receptor confer high resistance to fipronil in vivo. The observed resistance in A2'N mutants is likely attributable to a steric hindrance mechanism, wherein the introduction of larger side chains hampers fipronil binding, even in a heterozygous state. This article is protected by copyright. All rights reserved.