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Reaction injection molding of hydrophilic-in-hydrophobic femtolitre-well arrays.

Reza Zandi ShafaghDeborah DecropKaren VenArno VanderbekeRobert HanusaJolien BreukersGaspard PardonTommy HaraldssonJeroen LammertynWouter van der Wijngaart
Published in: Microsystems & nanoengineering (2019)
Patterning of micro- and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications, including cell-adhesion assays and highly sensitive bioassays. The manufacturing of such devices necessitates cumbersome multiple-step fabrication procedures and results in surface properties which degrade over time. This critically hinders their wide-spread dissemination. Here, we simultaneously mold and surface energy pattern microstructures in off-stoichiometric thiol-ene by area-selective monomer self-assembly in a rapid micro-reaction injection molding cycle. We replicated arrays of 1,843,650 hydrophilic-in-hydrophobic femtolitre-wells with long-term stable surface properties and magnetically trapped beads with 75% and 87.2% efficiency in single- and multiple-seeding events, respectively. These results form the basis for ultrasensitive digital biosensors, specifically, and for the fabrication of medical devices and life science research tools, generally.
Keyphrases
  • cell adhesion
  • public health
  • molecularly imprinted
  • liquid chromatography
  • ionic liquid
  • ultrasound guided
  • label free
  • high resolution
  • solid phase extraction
  • mass spectrometry