A fluorescence-based high-throughput screening method for cytokinin translocation mutants.

Mengyuan ZhangBingli DingJiangzhe ZhaoPenghong ZhangYujia LiGuodong YangKewei Zhang

Published in: Plant methods (2020)

We provide a strategy for screening mutants defective in cytokinin translocation, distribution or signaling. The strategy can be adapted to establish a system for screening mutants defective in other hormone transporters or signaling components using a fluorescence reporter.

Keyphrases

single molecule
wild type
energy transfer
crispr cas