Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications.

Peixin LiangYiting ZhangBo XuYuxin ZhaoXiangsheng LiuWeixia GaoTing MaChao YangShufang WangRuihua Liu

Published in: Microbial cell factories (2020)

These desirable traits make the GIs-deleted mutant KTU-U13 an optimum chassis for synthetic biology applications. The present study suggests that the systematic deletion of GIs in bacteria may be a useful approach for generating an optimal chassis for the construction of microbial cell factories.

Keyphrases

genome wide
single cell
cell therapy
copy number
gene expression
candida albicans