Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy.

Francesca PennacchiettiEkaterina O SerebrovskayaAline R FaroIrina I ShemyakinaNina G BozhanovaAlexey A KotlobayNadya G GurskayaAndreas BodénJes DreierDmitry M ChudakovKonstantin A LukyanovVladislav V VerkhushaAlexander S Mishin Ilaria Testa

Published in: Nature methods (2018)

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Keyphrases

living cells
fluorescent probe
single molecule
high resolution
quantum dots
deep learning
high speed
machine learning
photodynamic therapy
loop mediated isothermal amplification