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Correlation of Intravascular Ultrasound with Histology in Pediatric Pulmonary Vein Stenosis.

Ryan CallahanZachary GauthierShuhei TobaStephen P SandersDiego PorrasSara O Vargas
Published in: Children (Basel, Switzerland) (2021)
Preliminary intravascular ultrasound (IVUS) images of suspected pediatric intraluminal pulmonary vein stenosis (PVS) demonstrate wall thickening. It is unclear how the IVUS-delineated constituents of wall thickening correlate with the histology. We analyzed six postmortem formalin-fixed heart/lung specimens and four live patients with PVS as well as control pulmonary veins using IVUS and light microscopic examination. In PVS veins, IVUS demonstrated wall thickening with up to two layers of variable echogenicity, often with indistinct borders. Histologically, the veins showed fibroblastic proliferation with areas rich in myxoid matrix as well as areas with abundant collagen and elastic fibers. Discrete vein layers were obscured by scarring and elastic degeneration. A lower reflective periluminal layer by IVUS corresponded with hyperplasia of myofibroblast-like cells in abundant myxoid matrix. The hyper-reflective layer by IVUS extended to the outer edge of the vessel and corresponded to a less myxoid area with more collagen, smooth muscle and elastic fibers. The outer less reflective edge of the IVUS image correlated with a gradual transition into adventitia. Normal veins had a thin wall, correlating with histologically normal cellular and extracellular components, without intimal proliferation. IVUS may provide further understanding of the anatomy and mechanisms of pediatric pulmonary vein obstruction.
Keyphrases
  • smooth muscle
  • inferior vena cava
  • magnetic resonance imaging
  • coronary artery
  • deep learning
  • signaling pathway
  • heart failure
  • pulmonary hypertension
  • young adults
  • epithelial mesenchymal transition
  • machine learning