Target Recognition- and HCR Amplification-Induced In Situ Electrochemical Signal Probe Synthesis Strategy for Trace ctDNA Analysis.
Aiting CaiLuxia YangXiaoxia KangJinxia LiuFeng WangHaiwei JiQi WangMingmin WuGuo LiXiaobo ZhouYuling QinLi WuPublished in: Biosensors (2022)
An electrochemical-DNA (E-DNA) sensor was constructed by using DNA metallization to produce an electrochemical signal reporter in situ and hybridization chain reaction (HCR) as signal amplification strategy. The cyclic voltammetry (CV) technique was used to characterize the electrochemical solid-state Ag/AgCl process. Moreover, the enzyme cleavage technique was introduced to reduce background signals and further improve recognition accuracy. On the basis of these techniques, the as-prepared E-DNA sensor exhibited superior sensing performance for trace ctDNA analysis with a detection range of 0.5 fM to 10 pM and a detection limit of 7 aM. The proposed E-DNA sensor also displayed excellent selectivity, satisfied repeatability and stability, and had good recovery, all of which supports its potential applications for future clinical sample analysis.
Keyphrases
- circulating tumor
- label free
- nucleic acid
- single molecule
- cell free
- gold nanoparticles
- ionic liquid
- circulating tumor cells
- molecularly imprinted
- heavy metals
- solid state
- quantum dots
- particulate matter
- high resolution
- mass spectrometry
- air pollution
- highly efficient
- wastewater treatment
- current status
- real time pcr
- electron transfer
- endothelial cells
- data analysis