Iodine-124 Based Dual Positron Emission Tomography and Fluorescent Labeling Reagents for In Vivo Cell Tracking.
Truc Thuy PhamZhi LuChristopher DavisChun LiFangfang SunJohn MaherRan YanPublished in: Bioconjugate chemistry (2020)
Understanding the in vivo behavior of experimental therapeutic cells is fundamental to their successful development and clinical translation. Iodine-124 has the longest half-life (4.2 days) among the clinically used positron emitters. Consequently, this isotope offers the longest possible tracking time for directly labeled cells using positron emission tomography (PET). Herein, we have radiosynthesized and evaluated two iodine-124/fluorescein-based dual PET and fluorescent labeling reagents, namely 124I-FIT-Mal and 124I-FIT-(PhS)2Mal for cell surface thiol bioconjugation. 124I-FIT-(PhS)2Mal labeled cells significantly more effectively than 124I-FIT-Mal. It conjugated to various cell lines in 22%-62% labeling efficiencies with prolonged iodine-124 retention. 124I-FIT-(PhS)2Mal mainly conjugated on the cell membrane, which was confirmed by high-resolution fluorescence imaging. The migration of 124I-FIT-(PhS)2Mal labeled Jurkat cells was visualized in NSG mice with excellent target-to-background contrast using PET/CT over 7 days. These data demonstrate that 124I-FIT-(PhS)2Mal can dynamically track cell migration in vivo using PET/CT over a clinically relevant time frame.
Keyphrases
- positron emission tomography
- pet ct
- computed tomography
- induced apoptosis
- pet imaging
- cell cycle arrest
- high resolution
- cell migration
- endoplasmic reticulum stress
- oxidative stress
- dual energy
- type diabetes
- magnetic resonance imaging
- machine learning
- metabolic syndrome
- cell death
- stem cells
- single cell
- adipose tissue
- skeletal muscle
- high fat diet induced
- bone marrow
- contrast enhanced
- gas chromatography