Suncheonosides E-M and Benzothioate Derivatives from the Marine-Derived Streptomyces sp. ZSN77.

Thirteen new compounds, including suncheonosides E-M ( 1 - 9 ), four benzothioate derivatives ( 10 - 13 ), and one known compound ( 14 ), were identified from the marine-derived Streptomyces sp. ZSN77. Suncheonosides E-M incorporate β-d-glucose, while the reported suncheonosides (A-D) incorporate only l-rhamnose. All of the structures were determined by extensive analysis of NMR spectroscopic and HRESIMS data. Bioactivity evaluation of these compounds showed that 6 had significant activity against PC3 cells with an IC 50 value of 4.1 ± 0.1 μM, while compounds 12 and 14 exhibited cytotoxicity against HCT116 cells with IC 50 values of 7.3 ± 0.4 and 3.9 ± 0.3 μM, respectively. In addition, compounds 1 , 2 , 6 , 10 , and 14 displayed potent in vivo anti-inflammatory efficacy with inhibition of NO production in a dose-dependent manner.