A ZTF-7/RPS-2 complex mediates the cold-warm response in C. elegans.
Ting XuShimiao LiaoMeng HuangChengming ZhuXiaona HuangQile JinDemin XuChuanhai FuXiangyang ChenXuezhu FengShouhong GuangPublished in: PLoS genetics (2023)
Temperature greatly affects numerous biological processes in all organisms. How multicellular organisms respond to and are impacted by hypothermic stress remains elusive. Here, we found that cold-warm stimuli induced depletion of the RNA exosome complex in the nucleoli but enriched it in the nucleoplasm. To further understand the function and mechanism of cold-warm stimuli, we conducted forward genetic screening and identified ZTF-7, which is required for RNA exosome depletion from nucleoli upon transient cold-warm exposure in C. elegans. ZTF-7 is a putative ortholog of human ZNF277 that may contribute to language impairments. Immunoprecipitation followed by mass spectrometry (IP-MS) found that ZTF-7 interacted with RPS-2, which is a ribosomal protein of the small subunit and participates in pre-rRNA processing. A partial depletion of RPS-2 and other proteins of the small ribosomal subunit blocked the cold-warm stimuli-induced reduction of exosome subunits from the nucleoli. These results established a novel mechanism by which C. elegans responds to environmental cold-warm exposure.
Keyphrases
- mass spectrometry
- high glucose
- endothelial cells
- ms ms
- multiple sclerosis
- diabetic rats
- gene expression
- autism spectrum disorder
- genome wide
- drug induced
- oxidative stress
- small molecule
- amino acid
- capillary electrophoresis
- cerebral ischemia
- climate change
- human health
- gas chromatography
- protein protein
- heat stress
- life cycle