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Use of synthetic oligonucleotides for determination of HTLV-1 proviral load by real-time PCR: a helpful alternative approach in the clinical management.

Larissa Melo BandeiraMarco Antonio Moreira PugaVanessa Salete de PaulaLuiz Henrique Ferraz DemarchiG G C LichsJ A DomingosRivaldo Venâncio da CunhaS N O UeharaAna Rita Coimbra Motta Castro
Published in: Journal of applied microbiology (2020)
HTLV-1 PVL determination using synthetic oligonucleotides standard curve by qPCR could be a helpful alternative for the laboratories that monitor infected patients as an important prognostic factor in HTLV-1-associated diseases progression. Also, it can decrease costs and overcome the biological limitations of the plasmid curve.
Keyphrases
  • prognostic factors
  • real time pcr
  • solid phase extraction
  • molecularly imprinted
  • escherichia coli
  • nucleic acid
  • crispr cas
  • mass spectrometry
  • high resolution
  • simultaneous determination