Module Assembly Strategy for Single-Cell Nucleic Acid Imaging at the Sub-Molecule Level.
Xucong TengYicong DaiJinghong LiPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
Single-cell imaging has unique advantages of maintaining the in situ physiological state, morphology, and microenvironment, becoming a powerful tool to unravel the nature of intracellular nucleic acids. The analysis of nucleic acids unprecedentedly demands the sub-molecule details at segment or subunit, secondary structure and monomer levels, instead of just probing the sequence and the abundance of nucleic acids. Detection of nucleic acids at the sub-molecule level requires higher specificity and higher sensitivity, which becomes a new challenge in nucleic acid analysis. Herein, we summarize the recent progress in the design and the application of single-cell nucleic acid imaging methods at the sub-molecule level, including the visualization of RNA splicing variants, RNA G-quadruplexes in an individual gene, single nucleotide variation of mitochondrial DNA, and RNA m 6 A methylation. Remarkably, we highlight the key strategy, "Module Assembly", for high-performance molecular recognition and demonstrate the required improvements in future research.
Keyphrases
- nucleic acid
- single cell
- mitochondrial dna
- copy number
- high resolution
- rna seq
- high throughput
- genome wide
- stem cells
- dna methylation
- single molecule
- mass spectrometry
- molecular dynamics simulations
- transcription factor
- photodynamic therapy
- loop mediated isothermal amplification
- microbial community
- label free
- amino acid
- liquid chromatography
- sensitive detection
- tandem mass spectrometry
- simultaneous determination