Aptamer-17β-estradiol-antibody sandwich ELISA for determination of gynecological endocrine function.
Yue HuangLi ZhangZhenzuo LiSubash C B GopinathYeng ChenYan XiaoPublished in: Biotechnology and applied biochemistry (2020)
17β-Estradiol-E2 (17β-E2) is a steroid hormone that plays a major role in the reproductive endocrine system and is involved in various processes, such as pregnancy, fertility, and menopause. In this study, the performance of an enzyme-linked immunosorbent assay (ELISA) for 17β-E2 quantification was enhanced by using a gold nanoparticle (GNP)-conjugated aptamer. An anti-17β-E2-aptamer-GNP antibody was immobilized on an amine-modified ELISA surface. Then, 17β-E2 was allowed to interact with and be sandwiched by antibodies. Aptamer-GNP conjugation was confirmed by colorimetric assays via the naked eye and UV-visible light spectroscopy. The detection limit based on a signal-to-noise ratio (S/N) of 3 was estimated to be 1.5 nM (400 pg/mL), and the linear range was 1.5-50 nM. Control experiments (without 17β-E2/with a complementary aptamer sequence/with a nonimmune antibody) confirmed the specific detection of 17β-E2. Moreover, 17β-E2 spiking of human serum did not interrupt the interaction between 17β-E2 and its antibody and aptamer. Thus, the developed ELISA can be used as an alternate assay for quantification of 17β-E2 and assessment of endocrine-related gynecological problems.
Keyphrases
- gold nanoparticles
- label free
- sensitive detection
- magnetic nanoparticles
- loop mediated isothermal amplification
- high throughput
- photodynamic therapy
- visible light
- quantum dots
- monoclonal antibody
- mental health
- estrogen receptor
- high resolution
- postmenopausal women
- real time pcr
- pregnant women
- young adults
- preterm birth
- nitric oxide
- pregnancy outcomes
- single molecule
- simultaneous determination
- aqueous solution
- solid state