Single-cell sequencing in translational cancer research and challenges to meet clinical diagnostic needs.
Ulrich G PfistererJulia BräunigPer BrattåsMarkus HeidenbladGöran KarlssonThoas FioretosPublished in: Genes, chromosomes & cancer (2021)
The ability to capture alterations in the genome or transcriptome by next-generation sequencing has provided critical insight into molecular changes and programs underlying cancer biology. With the rapid technological development in single-cell sequencing, it has become possible to study individual cells at the transcriptional, genetic, epigenetic, and protein level. Using single-cell analysis, an increased resolution of fundamental processes underlying cancer development is obtained, providing comprehensive insights otherwise lost by sequencing of entire (bulk) samples, in which molecular signatures of individual cells are averaged across the entire cell population. Here, we provide a concise overview on the application of single-cell analysis of different modalities within cancer research by highlighting key articles of their respective fields. We furthermore examine the potential of existing technologies to meet clinical diagnostic needs and discuss current challenges associated with this translation.
Keyphrases
- single cell
- rna seq
- papillary thyroid
- high throughput
- squamous cell
- induced apoptosis
- gene expression
- genome wide
- dna methylation
- single molecule
- small molecule
- young adults
- cell cycle arrest
- mesenchymal stem cells
- childhood cancer
- amino acid
- bone marrow
- cell death
- risk assessment
- oxidative stress
- binding protein
- heat shock protein
- endoplasmic reticulum stress
- sensitive detection
- heat shock