In vivo mRNA imaging based on tripartite DNA probe mediated catalyzed hairpin assembly.
Han WuWen-Jing ZhouLan LiuZe FanHao TangRu-Qin YuJian-Hui JiangPublished in: Chemical communications (Cambridge, England) (2021)
Herein, we develop a novel tripartite DNA probe to transport phosphorothioated substrate hairpins and an aptamer for the intramolecular CHA circuit, which achieves detection of a low amount of specific mRNA in living cells and mice. Our study provides an improved strategy to promote in vivo fluorescence imaging applications in early-stage clinical diagnosis.
Keyphrases
- living cells
- single molecule
- fluorescence imaging
- fluorescent probe
- early stage
- circulating tumor
- photodynamic therapy
- cell free
- label free
- gold nanoparticles
- binding protein
- room temperature
- nucleic acid
- loop mediated isothermal amplification
- high fat diet induced
- sensitive detection
- skeletal muscle
- sentinel lymph node
- squamous cell carcinoma
- neoadjuvant chemotherapy
- radiation therapy
- circulating tumor cells
- adipose tissue
- amino acid
- lymph node
- locally advanced