Next-generation sequencing of PTEN mutations for monitoring minimal residual disease in T-cell acute lymphoblastic leukemia.
Giuseppe GermanoMaria Grazia ValsecchiBarbara BuldiniGiovanni CazzanigaCarlo ZanonDaniela SilvestriGeertruy Te KronnieGiuseppe BassoMaddalena PaganinPublished in: Pediatric blood & cancer (2019)
Minimal residual disease (MRD) analysis has become a powerful indicator to refine therapy in acute lymphoblastic leukemia (ALL). Here, we present an MRD detection based on the next-generation sequencing of PTEN exon 7 mutations (NGS-PTEN) in 30 pediatric T-cell ALL patients. By comparing the NGS-PTEN results with current quantitative PCR of antigen receptor gene rearrangements (qPCR-Ig/TR), an overall concordance of 80% was found between the two methods. However, the NGS-PTEN qualified a lower number of high-risk patients than qPCR-Ig/TR. These findings suggest that NGS-PTEN is a promising tool that could potentially be used to support current MRD methodologies for T-ALL patients.
Keyphrases
- acute lymphoblastic leukemia
- end stage renal disease
- cell proliferation
- ejection fraction
- newly diagnosed
- chronic kidney disease
- pi k akt
- prognostic factors
- peritoneal dialysis
- gene expression
- high resolution
- acute myeloid leukemia
- mass spectrometry
- dna methylation
- allogeneic hematopoietic stem cell transplantation
- genome wide
- binding protein
- smoking cessation