Widefield in vivo imaging system with two fluorescence and two reflectance channels, a single sCMOS detector, and shielded illumination.

Patrick R DoranNatalie Fomin-ThunemannRockwell P TangDora BalogBernhard ZimmermanKıvılcım KılıçEmily A MartinSreekanth KuraHarrison P FisherGrace ChabbottJoel HerbertBradley C RauscherJohn X JiangSava SakadzicDavid A Boas Anna Devor Ichun Anderson Chen Martin Thunemann

Published in: Neurophotonics (2024)

Our widefield microscope design with a single camera can be used to acquire multiple aspects of brain physiology and is compatible with behavioral readouts of pupil diameter.

Keyphrases

high resolution
resting state
white matter
single molecule
high speed
functional connectivity
convolutional neural network
cerebral ischemia
energy transfer
computed tomography
fluorescence imaging
brain injury
mass spectrometry
subarachnoid hemorrhage
deep learning