Drug Resistance Reversal Potential of Nanoparticles/Nanocomposites via Antibiotic's Potentiation in Multi Drug Resistant P. aeruginosa .
Pratima PandeyRajashree SahooKhusbu SinghSanghamitra PatiJose MathewAvinash Chandra PandeyRajni KantIhn HanEun-Ha ChoiGaurav Raj DwivediDharmendra Kumar YadavPublished in: Nanomaterials (Basel, Switzerland) (2021)
Bacteria employ numerous resistance mechanisms against structurally distinct drugs by the process of multidrug resistance. A study was planned to discover the antibacterial potential of a graphene oxide nanosheet (GO), a graphene oxide-zinc oxide nanocomposite (GO/ZnO), a graphene oxide-chitosan nanocomposite (GO-CS), a zinc oxide decorated graphene oxide-chitosan nanocomposite (GO-CS/ZnO), and zinc oxide nanoparticles (ZnO) alone and in a blend with antibiotics against a PS-2 isolate of Pseudomonas aeruginosa . These nanocomposites reduced the MIC of tetracycline (TET) from 16 folds to 64 folds against a multidrug-resistant clinical isolate. Efflux pumps were interfered, as evident by an ethidium bromide synergy study with nanocomposites, as well as inhibiting biofilm synthesis. These nanoparticles/nanocomposites also decreased the mutant prevention concentration (MPC) of TET. To the best of our knowledge, this is the first report on nanomaterials as a synergistic agent via inhibition of efflux and biofilm synthesis.
Keyphrases
- reduced graphene oxide
- oxide nanoparticles
- drug resistant
- pseudomonas aeruginosa
- multidrug resistant
- visible light
- acinetobacter baumannii
- gold nanoparticles
- biofilm formation
- drug delivery
- staphylococcus aureus
- cystic fibrosis
- gram negative
- healthcare
- candida albicans
- klebsiella pneumoniae
- cancer therapy
- climate change
- hyaluronic acid
- silver nanoparticles
- room temperature
- high resolution
- solid phase extraction