Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples.

Hui-Qi QuCharlly KaoJames GarifallouFengxiang WangJames SnyderDiana J SlaterCuiping HouMichael E MarchJohn J ConnollyJoseph T GlessnerHakon Hakonarson

Published in: Genes (2023)

Significant transcriptome changes were observed across different PBMC cell types as a factor of time from blood draw to PBMC isolation and as a consequence of blood storage. This should be kept in mind when interpreting experimental results.

Keyphrases

single cell
rna seq
high throughput
gene expression
genome wide
cell therapy
room temperature