Heterogeneous loss of HIV transcription and proviral DNA from 8E5/LAV lymphoblastic leukemia cells revealed by RNA FISH:FLOW analyses.
Kaley M WilburnHenry C MwandumbaKondwani C JamboSaikat BoliarSabrina SoloukiDavid G RussellDavid W GludishPublished in: Retrovirology (2016)
8E5/LAV cells harbor a single HIV provirus, and are used frequently to generate standards for HIV genome quantification. Using flow cytometry-based in situ mRNA hybridization validated by qPCR, we find that different batches of 8E5 cells contain varying numbers of cells lacking viral mRNA and/or viral genomes. These findings raise concerns for studies employing 8E5 cells for quantitation, and highlight the value of mRNA FISH and flow cytometry in the detection and enumeration of HIV-positive cells.
Keyphrases
- induced apoptosis
- hiv positive
- cell cycle arrest
- antiretroviral therapy
- flow cytometry
- human immunodeficiency virus
- hiv infected
- endoplasmic reticulum stress
- sars cov
- oxidative stress
- signaling pathway
- bone marrow
- transcription factor
- ms ms
- high resolution
- genome wide
- quantum dots
- binding protein
- high performance liquid chromatography
- liquid chromatography