NPs-TiO2 and Lincomycin Coexposure Induces DNA Damage in Cultured Human Amniotic Cells.
Filomena MottolaConcetta IovineMarianna SantonastasoMaria Luisa RomeoSeverina PacificoLuigi CobellisLucia RoccoPublished in: Nanomaterials (Basel, Switzerland) (2019)
Titanium dioxide nanoparticles (NPs-TiO2 or TiO2-NPs) have been employed in many commercial products such as medicines, foods and cosmetics. TiO2-NPs are able to carry antibiotics to target cells enhancing the antimicrobial efficiency; so that these nanoparticles are generally used in antibiotic capsules, like lincomycin, added as a dye. Lincomycin is usually used to treat pregnancy bacterial vaginosis and its combination with TiO2-NPs arises questions on the potential effects on fetus health. This study investigated the potential impact of TiO2-NPs and lincomycin co-exposure on human amniocytes in vitro. Cytotoxicity was evaluated with trypan blue vitality test, while genotoxic damage was performed by Comet Test, Diffusion Assay and RAPD-PCR for 48 and 72 exposure hours. Lincomycin exposure produced no genotoxic effects on amniotic cells, instead, the TiO2-NPs exposure induced genotoxicity. TiO2-NPs and lincomycin co-exposure caused significant increase of DNA fragmentation, apoptosis and DNA damage in amniocytes starting from 48 exposure hours. These results contribute to monitor the use of TiO2-NPs combined with drugs in medical application. The potential impact of antibiotics with TiO2-NPs during pregnancy could be associated with adverse effects on embryo DNA. The use of nanomaterials in drugs formulation should be strictly controlled in order to minimize risks.
Keyphrases
- visible light
- quantum dots
- dna damage
- oxide nanoparticles
- cell cycle arrest
- induced apoptosis
- oxidative stress
- endothelial cells
- healthcare
- human health
- endoplasmic reticulum stress
- dna repair
- cell free
- cell death
- drug delivery
- risk assessment
- staphylococcus aureus
- mesenchymal stem cells
- cell proliferation
- circulating tumor
- signaling pathway
- drug induced
- high glucose
- pregnant women
- diabetic rats
- health information
- social media
- climate change
- bone marrow
- mass spectrometry
- preterm birth
- pregnancy outcomes
- highly efficient